Flow Cytometry
The Flow Cytometry Unit offers 4 analyzers, 3 sorters, and 3 workstations with flow analysis software: FCS Express and FLOWjo.
All users receive full training and guided assistance on the various instruments.
Workshops and hands-on sessions are organized to train researchers in flow cytometry methods and advanced applications and protocols.
The flow cytometry unit contains the following equipment:
Sorters
Bigfoot cell sorter is a new digital flexible and stable stream-in-air flow cytometry sorter, configured with 5 100mW air-cooled lasers at excitations of 355nm, 405nm, 488nm, 561nm, and 640nm and up to 48 fluorescent detectors with spectral unmixing ability.
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BD FACS Aria-IIIu cell sorter is a new digital sorter enabling high-speed sorting and multicolor analysis.
The instrument accommodates 4 air-cooled lasers at 407 nm, 488nm, 651nm, and 633nm.
Analyzers
The BD LSR-II is a flow cytometer equipped with 4 lasers. The cytometer allows the detection of 12 parameters simultaneously (10 colors plus forward scatter and side scatter).
The LSR-II offers high acquisition speeds, a full inter- and intra-laser compensation matrix, and multiple thresholds on all lasers.
The BD LSR-II SORP is an analyzer flow cytometer equipped with 4 lasers.
The cytometer allows the detection of 15 parameters simultaneously (15 colors plus forward scatter and side scatter).
The Cytek Amnis ImageStream®X Mark II is an imaging flow cytometer equipped with 4 lasers. Imaging Flow Cytometry combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy.
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Cytek Aurora is a spectral flow cytometer equipped with four lasers (405, 488, 561, and 635 nm). Aurora’s advanced technology allows the detection of the full emission spectrum, which enables it to distinguish between overlapping dyes such as GFP and FITC or APC and AF 647.
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The BD FACS Calibur is an analog analyzer equipped with 2 lasers permitting the detection of 4 colors. The main laser is an air-cooled Argon Ion laser exciting at 488 nm (15mW) and the second laser is a Red Diode exciting at 635 nm (9mW).
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