
Nikon- Spinning Disk Confocal

Description
The Spinning Disk Confocal microscope from Nikon with CSU-W1 Confocal Scanner Unit with dual camera from Yokogawa, this system is designed for live-cell imaging.
It is hooked to an inverted fully motorized Ti2-E microscope stand.
Features
SYSTEM SPECIFICATIONS:
Imaging: Fast imaging with low phototoxicity;
Stand: Nikon Ti2-E
XY-stage: motorized
Z – Piezo focus NIDAQ 200 um range
Perfect focus
Sola LED illumination
Equipped for live imaging (temperature and CO2 incubation)
Software: NIS with image intelligence module
LASER OPTIONS:
The system includes 4 visible solid state lasers:
- UV diode laser – 405 nm (120mW)
- Blue diode laser 488 (200mW)
- Green diode laser – 561 nm (150mW)
- Red diode laser – 640 nm (200mW)
CAMERA SPECIFICATIONS:
Model: Two Photometrics BSI, sCMOS
Effective number of pixels: 2048 x 2048
Pixel size: 6.5µm x 6.5µm
Effective Area: 13.312mm x 13.312mm
Dynamic range: 16 bit
Max frame rate: 43 fps @ 16-bit/ 12-bit (CMS)
63 fps @ 11-bit
Quantum efficiency (peak): > 95% (@560nm)
Read out noise: 1.0e- Read Noise with Correlated Multi Sampling (CMS) (depending on fps)
Spectrum Capabilities UV (200-400 nm), Visible (400-700 nm), Near Infrared (700-1000 nm)
Objectives
Magnitude | Name | Serial # | NA | Immersion | Working Distance (um) |
---|---|---|---|---|---|
2X | CFI PLAN APOCHROMAT LAMBDA D | MRD07001 | 0.1 | DRY | 8500 |
10X | CFI PLAN APOCHROMAT LAMBDA | MRD00105 | 0.45 | DRY | 4000 |
20X | CFI S PLAN FLUOR LWD | 0.7 | DRY | 2300 | |
40X | CFI PLAN APOCHROMAT LAMBDA | MRD00405 | 0.95 | DRY | 130 |
60X | CFI PLAN APO VC | MRD07602 | 1.2 | Water | 250 |
60X -TIRF | CFI APOCHROMAT TIRF 60HX | 1.49 | Oil | 130 | |
100X | CFI SR HP PLAN APOCHROMAT LAMBDA S | 1.35 | Silicone | 310 | |
100X | CFI PLAN APOCHROMAT | 1.45 | Oil | 230 |
FILTERCUBES
LED FILTER | DAPI | FITC | TRITC | Cy5 |
---|---|---|---|---|
Ex | 392/23 | 474/27 | 554/23 | 635/18 |
DM | 409 | 495 | 573 | 652 |
EM | 447/60 | 525/45 | 609/54 | 680/42 |
CAMERA FILTERS
CAMERA | DAPI | FITC | TRITC | Cy5 |
---|---|---|---|---|
MASTER | 447/60 | 525/50 | 600/52 | 708/75 |
SLAVE | — | — | 600/52 | 708/75 |
Dichroic Mirror | for Dual camera | LP561 |
Applications
The system enables, amongst many others, the following applications:
- Fast Image Acquisition for Live Cell Imaging and Time-Lapse studies,
- Z-Stacks for localization of cells in living tissues and 3-D reconstruction
- High-resolution localization of sub-cellular compartments and quantities co-localization
- FRET – fluorescence resonance energy transfer
- Mark and find feature – The motorized stage allows marking specific coordinates using small magnifications or tiling of large scanning areas for further detailed scanning.
- Tracking module
- JOBS and AI tool
For booking or more info
Please contact Microscopy Team,
Tel. 073-378-1385/6, to coordinate a meeting.
