LSM 710 – Inverted Confocal
Description
The LSM 710 laser scanning confocal microscope from Zeiss is a spectral imaging confocal system designed for live-cell imaging. It is hooked to an inverted fully motorized Axio Observer Z1 microscope.
Features
Laser options:
The system includes 4 visible solid state lasers:
- UV laser – 405 nm (10mW)
- Argon multi line laser – 458, 488 and 514 nm (30mW)
- Green laser – 543 nm (10mW)
- Red laser – 639 nm (5mW)
Objectives
| Magnitude | Objective | Serial # | NA | Immersion | Contrast |
|---|---|---|---|---|---|
| 10X | EC Plan-Neofluar | 420340-9901-000 | 0.3 | DRY | DIC I |
| 20X | Plan-Apochromat | 420650-9901-000 | 0.8 | DRY | DIC II |
| 40X | C-ApoChromat | 421767-9970-000 | 1.2 | Water (correction ring) | DIC III |
| 63X | Plan-Apochromat | 420782-9900-000 | 1.4 | Oil | DIC III |
- Controller: Two-mirror scanner system offers 360° XY scanning field rotation, quick zooming, different acquisition modes: simultaneous, sequential, bi-directional and more.
- Detection: The scanning module features a confocal beam path with two channels and continuously variable secondary dichroic beam splitter for free selection of emission bands; 2 symmetric galvanometer mirrors for high-precision beam positioning; One motorized and continuously variable high precision pinhole; Two PMTs (photomultipliers) detectors and Two highly sensitive GaAsp (Gallium arsenide phosphide) detectors. In addition, the system provides transmission images via the Transmission Photomultiplier.
- Incubation: This microscope has an incubation chamber for live cell imaging for control over CO2, temperature and humidity.
Applications
The system enables, amongst many others, the following applications:
- Photoactivation and Photobleaching experiments
- Fast Image Acquisition for Live Cell Imaging and Time-Lapse studies
- Z-Stacks for localization of cells in living tissues and 3-D reconstruction
- High-resolution localization of sub-cellular compartments and quantities co-localization
- Spectral imaging
- FRET – Fluorescence Resonance Energy Transfer
- FRAP - Fluorescence Recovery After Photo-bleaching
- Mark and find feature – The motorized stage allows marking specific coordinates using small magnifications or tiling of large scanning areas for further detailed scanning.
For booking or more info
Please contact Microscopy Team,
Tel. 073-378-1385/6, to coordinate a meeting.
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