FACS Aria–IIIu Sorter
Description
BD FACSAria-IIIu cell sorter is a new digital sorter enabling high-speed sorting and multicolor analysis.
The instrument accommodates 4 air-cooled lasers at 407 nm, 488nm, 651nm, and 633nm.
Sorting at up to 15,000 events per second
Multicolor analysis of up to 15 parameters
Up to four-way bulk sorting devices for a variety of tube sizes
Two-way 50ml bulk sorting
Automated Cell Deposition Unit (ACDU) for sorting into multi-well plates or microscope slides
Features
Optical System: Patented BD OctagonTM and TrigonTM technology.
Laser excitation and filters (see FACSAria III configuration in the related links):
Lasers: 407 nm, 488nm, 561nm, and 633nm.
15 parameters: forward scatter, side scatter, 2 fluorescent detectors of 407nm, 5 fluorescent detectors of 488nm, 4 fluorescent detectors of 561nm, and 2 fluorescent detectors of 633nm.
Specifications and performance
Fluorescence Sensitivity
FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)
Fluorescence Resolution
Coefficient of variation PI-Area of <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei
Coefficient of variation Hoechst-Area of <3.5%, full G0/G1 peak for Hoechst-stained chicken erythrocyte nuclei
Fluorescence Linearity
Doublet/singlets ratio for CEN stained with PI = 1.95-2.05 detected off the 488-nm laser or Hoechst = 1.95-2.05 detected off the 407-nm laser
Forward and Side Scatter Sensitivity
Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and 0.5-micron beads.
Forward and Side Scatter Resolution
Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.
Sample Acquisition Rate
Maximum acquisition rate: 70,000 events per second with 8 parameters, 12 compensation pairs. Maximum rate < 70,000 events per second with more parameters, > 100,000 events per second with fewer parameters.
Sorting options:
Two- and four-way sorting into microtubes, 5ml, 15ml, and 50ml tubes
ACDU (automated cell deposition unit) for sorting into multi-well plates and onto microscope slides
Sample injection chamber for various sample input tubes, including microtubes, 5ml, 15ml, and 50ml tubes. Includes sample agitation and temperature control.
Signal Processing
- Digital electronics with 10 MHz sampling frequency
- Up to sixteen acquisition channels with up to four spatially separated laser beams
- 18-bit data acquisition for area signals
- Inter- and intra-beam compensation on-line and post-acquisition off-line
- Height, width, and area measurements available for all parameters
- Time parameter available for kinetic studies (Calcium)
- Ratio measurements between any two parameters from any laser
Nozzle selection and design
An expanded set of nozzles lets users sort a wide range of particle sizes. Four nozzle sizes are supported: 70, 85, 100, and 130 microns.
Working policy
Users who wish to work on the FACS-Aria-IIIu must contact Dr. Aviv Lutaty before ordering this system.
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