FACS Aria–IIIu Sorter

Description

BD FACSAria-IIIu cell sorter is a new digital sorter enabling high-speed sorting and multicolor analysis.
The instrument accommodates 4 air-cooled lasers at 407 nm, 488nm, 651nm, and 633nm.
Sorting at up to 15,000 events per second
Multicolor analysis of up to 15 parameters
Up to four-way bulk sorting devices for a variety of tube sizes
Two-way 50ml bulk sorting
Automated Cell Deposition Unit (ACDU) for sorting into multi-well plates or microscope slides

Features

Optical System: Patented BD OctagonTM and TrigonTM technology.
Laser excitation and filters (see FACSAria III configuration in the related links):
Lasers: 407 nm, 488nm, 561nm, and 633nm.
15 parameters: forward scatter, side scatter, 2 fluorescent detectors of 407nm, 5 fluorescent detectors of 488nm, 4 fluorescent detectors of 561nm, and 2 fluorescent detectors of 633nm.

 

FACS Aria–IIIu

Lasers and detectors:

Laser line/color 
(Excitation)		DetectorEmission Filter (nm)Example of available markers
407 nm  Violet  A  695 /40 (Far red) QD-710 
 B   450/40 (Blue) DAPI, Hoechst 33258, Pacific Blue, BV421, Alexa Fluor 405 
488 nm  Blue  A  780 / 60 (Infra-Red)  PE-Cy7 
 B   695 / 40 (Red-Far-Red) PE-Cy5, PerCP, PerCP-Cy5.5, 7-AAD, PI 
 C 616/23 (Red) PE-Texas Red, PI 
 D   585 / 42 (Orange-Red) PE, PI 
  E 530 / 30 (Green) Alexa Fluor 488, FITC, GFP, CFSE, Sytox Green, YFP 
F  488 / 10 SSC 
   FSC 
561 nm  Yellow Green  A  780 / 60 (Infra-Red)  PE-Cy7 
 B 670/14 (Far Red) 7-AAD , PE-Cy5 
 C 610/20 (Red) mCherry, PE-CF594 , PI 
 D 582/15 (Orange-Red) Cy3, PE 
633nm  Red  A 780/60 (Infra-Red) APC-Cy7 
 B   660/20 (Red) APC 

Additional available filters:

Mirror 450LP 5005LP 530LP 635LP 685LP 735LP   
Bandpass 405/20 525/50 530/30 585/42 610/20 670/14 675/20 695/40 

Specifications and performance

Fluorescence Sensitivity

FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)

PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

Coefficient of variation PI-Area of <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei

Coefficient of variation Hoechst-Area of <3.5%, full G0/G1 peak for Hoechst-stained chicken erythrocyte nuclei

Fluorescence Linearity

Doublet/singlets ratio for CEN stained with PI = 1.95-2.05 detected off the 488-nm laser or Hoechst = 1.95-2.05 detected off the 407-nm laser

Forward and Side Scatter Sensitivity

Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and 0.5-micron beads.

Forward and Side Scatter Resolution

Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

Maximum acquisition rate: 70,000 events per second with 8 parameters, 12 compensation pairs. Maximum rate < 70,000 events per second with more parameters, > 100,000 events per second with fewer parameters.

Sorting options:

Two- and four-way sorting into microtubes, 5ml, 15ml, and 50ml tubes

ACDU (automated cell deposition unit) for sorting into multi-well plates and onto microscope slides

Sample injection chamber for various sample input tubes, including microtubes, 5ml, 15ml, and 50ml tubes. Includes sample agitation and temperature control.

Signal Processing

  • Digital electronics with 10 MHz sampling frequency
  • Up to sixteen acquisition channels with up to four spatially separated laser beams
  • 18-bit data acquisition for area signals
  • Inter- and intra-beam compensation on-line and post-acquisition off-line
  • Height, width, and area measurements available for all parameters
  • Time parameter available for kinetic studies (Calcium)
  • Ratio measurements between any two parameters from any laser

Nozzle selection and design

An expanded set of nozzles lets users sort a wide range of particle sizes. Four nozzle sizes are supported: 70, 85, 100, and 130 microns.

Working policy

Users who wish to work on the FACS-Aria-IIIu must contact Dr. Aviv Lutaty  before ordering this system.

Related Documents

AriaIII configuration