Bigfoot Cell Sorter
Description
Bigfoot cell sorter is a new digital flexible and stable stream-in-air flow cytometry sorter, configured with 5 100mW air-cooled lasers at excitations of 355nm, 405nm, 488nm, 561nm, and 640nm and up to 48 fluorescent detectors with spectral unmixing ability.
Features
Sorting options:
- 6-way sorting into tubes (200ul, 1.5ml, 5ml, 15ml or 50 ml),
- 4-way sorting into 96-well plates (in less than 8 seconds),
- 8-way sorting into 384-well plates (in less than 11 seconds),
- or straight down sorting into 1536-well plates.
- In addition, virtual 18-way sorting allows the separation of multiple populations from a single sample or different samples.
- For bulk sorts, the InfiniSort capability allows multiple plate sorts in sequence.
Sample input tube types
1.5 mL, 5 mL, and 15 mL
Sample output tube types
- 200ul, 1.5ml, 5ml, 15ml or 50 ml, tubes and 96, 384 and 1536 well plates
- Adaptor for 10x genomic chip
- Adaptor for slides
- Adaptor for PCR strips
- Adaptor for 10cm plates
Beam alignment
Fixed, 7 spatially separated pinholes
Scatter parameters
Standard FSC and SSC 488 nm; small particle FSC 405 nm; polarized FSC and SSC 488nm
Scatter resolution
<0.2 µm scatter resolution from a background with a small particle detector
Pulse measurement
Simultaneously measured peak, area, and width for sample input and output of every channel.
Sample Acquisition Rate
Maximum acquisition rate: >100,000 events per second with 48 parameters.
Temperature control
4°C-37°C for input and output of all media types.
Agitation
Automatically based on the input tube type.
Nozzle selection and design
Four nozzle sizes are supported: 70, 100, 120, and 150 microns with adjustable pressure settings.
Specifications and performance
Integrated biocontainment and aerosol management
The Bigfoot Spectral Cell Sorter features an integrated Class II biocontainment cabinet, which meets the equivalent functional personnel and product protection standards for a Class II Type A2 biosafety cabinet defined by the National Sanitation Foundation International Standard 49. The custom-designed cabinet protects operators and samples from aerosols without compromising high-parameter sorter performance or impacting workflow. A separate aerosol management system meets ISAC guidelines for cell sorters.
Working policy
Users who wish to work on the Bigfoot system must contact Dr. Aviv Lutaty before ordering this system.