FACS Calibur Analyzer
Description
The BD FACS Calibur is an analog analyzer equipped with 2 lasers permitting the detection of 4 colors. The main laser is an air-cooled Argon Ion laser exciting at 488 nm (15mW) and the second laser is a Red Diode exciting at 635 nm (9mW).
Features
FACS Calibur can measure the following parameters (see configuration):
- Forward Scatter (FSC) expresses the size of a cell.
- Side Scatter (SSC) expresses the granularity of a cell.
Fluorescent parameters:
- FL1 – Green emission (filter: 530/30)
- FL2 – Red/Orange emission (filter: 585/42)
- FL3 – Red emission (filter: 670 LP)
- FL4 – Far Red emission (filter: APC – 661/16 nm)
Specifications and performance
Fluorescence sensitivity: 1000 molecules of equivalent soluble fluorescein.
Fluorescence resolution: Coefficient of variation (CV) is <3% full peak for propidium iodide-stained (PI) chicken erythrocyte nuclei (CEN) for FL2-A.
Flow rates
Low: 12µl/min.
Med: 35µl/min.
High: 60µl/min.
Applications
Analysis of particles of sizes ranging from 0.2-150 microns
Cell cycle and DNA analysis
1-4 colors analysis
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