BD LSR-II SORP Analyzer

Description

The BD LSR-II SORP is an analyzer flow cytometer equipped with 4 lasers.

The cytometer allows the detection of 15 parameters simultaneously (15 colors plus forward scatter and side scatter).

The LSR-II SORP offers high acquisition speeds, full inter- and intra-laser compensation matrix, and multiple thresholds on all lasers. Digital pulse sampling allows the measurement of Height, Area, and Width from all parameters simultaneously.

Features

  • Optical System: Patented BD OctagonTM and TrigonTM technology. A quartz cuvette is coupled to the emission lens by refractive index-matching optical gel for optimum collection efficiency.
  • Laser excitation and filters (see LSR-II configuration in the related links): 

Lasers and Detectors:

Lasers and Detectors
Laser line/color 
(Excitation) 		Detector Emission Filter (nm) Example of available markers 
  Violet (405nm)  A 780 / 60 – 740 BV750, BV785 
 B   705 / 70 – 685LP BV711 , BV650 
 C 610 / 20 – 595LP BV605 
 D 525 / 50 – 505LP BV510, Zombie Aqua 
  E 450 / 50 Pacific Blue, BV421, DAPI, Hoechst, CFP 
Blue (488nm)  A 710 / 50 – 685LP PerCP, PerCP-Cy5-5, 7-AAD 
 B 525 / 50 – 505LP GFP, AF488, YFP 
 C 488 / 10 SSC 
 D  FSC 
Yellow/green (561nm)  A 780 / 60 – 740LP PE-Cy7 
 B 710 / 50 – 685LP PE-Cy5.5 
 C 660 / 20 – 635LP PE-Cy5 
 D 610 / 20 – 600LP mCherry, PI 
 E 582 / 15 -570LP DsRed, PE 
Red (640nm)  A 780 / 60 – 740LP APC- cy7 
 B 710 / 50 – 685LP Vio Bright R720, AF700 
 C 670 / 30 AF647, APC 

Additional available filters:

Mirror 450LP 5005LP 530LP 635LP 685LP 735LP
Bandpass 405/20 525/50 530/30 585/42 610/20 670/14 675/20 695/40

 

  • High Throughput System (HTS) – Enables analysis directly from 96 multi-well plates. The workstation is managed by FACS-Diva 6.1 software.

 

Acquisition Rate: >20000 events per second

Fluorescence Sensitivity: The detection limit is <200 molecules of equivalent soluble fluorochrome (MESF).

Fluorescence Resolution: The coefficient of variation (CV) of FL-Area is <3%, determined by full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN Quality Control Kit).

Fluorescence Linearity: Doublet/singlet ratio for CENs stained with PI or Hoechst is 1.95-2.05, detected with a 488 nm laser.

Forward and Side Scatter Sensitivity: Sensitivity enables the separation of fixed platelets from noise.

Signal Processing

  • Digital electronics with 10 MHz sampling frequency
  • 18-bit data acquisition for area signals
  • Inter- and intra-beam compensation on-line and post-acquisition off-line
  • Height, Width, and Area measurement available for all parameters
  • Time parameter available for kinetic studies (Calcium)
  • Ratio measurements between any two parameters from any laser

Software

  • BD FACSDiVaTM Software Version 6.1
  • All data are saved linearly
  • Complex hierarchical gating for multicolor applications
  • Automatic compensation of all parameters after acquisition
  • Complete set of statistics for all analysis

Applications

Analysis of cells, yeast, bacteria, and beads

  • Multi-color analysis
  • HTS system
  • Calcium Flux
  • DNA analysis

Related Documents

BD LSR-II User Guide

BD LSR-II SORP Configuration