BD LSR-II Analyzer

Description

The BD LSR-II is a flow cytometer equipped with 4 lasers. The cytometer allows the detection of 12 parameters simultaneously (10 colors plus forward scatter and side scatter).

The LSR-II offers high acquisition speeds, a full inter- and intra-laser compensation matrix, and multiple thresholds on all lasers. Digital pulse sampling allows the measurement of Height, Area, and Width from all parameters simultaneously.

Features

  • Optical System: Patented BD OctagonTM and TrigonTM technology. A quartz cuvette is coupled to the emission lens by refractive index-matching optical gel for optimum collection efficiency.
  • Laser excitation and filters (see LSR-II configuration in the related links):

Lasers and Detectors:

Lasers and Detectors
Laser line/color 
(Excitation) 		Detector Emission Filter (nm) Example of available markers 
488 nm  Blue  A  780 / 60 (Infra-Red)  PE-Cy7 
 B   695 / 40 (Red-Far-Red) PE-Cy5, PerCP, PerCP-Cy5-5, 
  7-AAD 
 C  575 / 26 (Orange-Red)  PE, PI, PKH26 
 D   530 / 30 (Green) Alexa Four 488, FITC, GFP,  CFSE, Sytox Green, YFP, 
  E  488 / 10 (Blue)  SSC 
   FSC 
633 nm Red  A   780 / 60 (Infra Red)   APC-Cy7, APC-H7 
 B  660 / 20 (Red)  APC, Alexa Flour 647 
405 nm  Violet  A   695 / 40 (Infra Red)  BV650, BV711 
 B   450 / 50 (Blue)  Pacific Blue, BV421 
355 nm  UV  A  530 / 30 (Green)   Indo 1 (Blue, Calcium Flux) 
 B  450 / 50 (Blue)  DAPI , Hoechst, Indo 1 (Violet) 
  • High Throughput System (HTS) – Enables analysis directly from 96 multi-well plates. The workstation is managed by FACS-Diva 6.1 software.

 

Additional available filters:

Mirror 450LP5005LP 530LP 635LP 685LP 735LP   
Bandpass 405/20 525/50 530/30 585/42 610/20 670/14 675/20 695/40 

 

Acquisition Rate: >20000 events per second
Fluorescence Sensitivity: The detection limit is <200 molecules of equivalent soluble fluorochrome (MESF).
Fluorescence Resolution: The coefficient of variation (CV) of FL-Area is <3%, determined by full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN Quality Control Kit).

Fluorescence Linearity: Doublet/singlet ratio for CENs stained with PI or Hoechst is 1.95-2.05, detected with 488nm or UV laser.

Forward and Side Scatter Sensitivity: Sensitivity enables the separation of fixed platelets from noise.

Signal Processing

  • Digital electronics with 10 MHz sampling frequency
  • 18-bit data acquisition for area signals
  • Inter- and intra-beam compensation on-line and post-acquisition off-line
  • Height, Width, and Area measurement available for all parameters
  • Time parameter available for kinetic studies (Calcium)
  • Ratio measurements between any two parameters from any laser

Software

  • BD FACSDiVaTM Software Version 6.1
  • All data are saved linearly
  • Complex hierarchical gating for multicolor applications
  • Automatic compensation of all parameters after acquisition
  • Complete set of statistics for all analysis

Applications

Analysis of cells, yeast, bacteria, and beads

  • Multi-color analysis
  • HTS system
  • Calcium Flux
  • DNA analysis

 

Related Documents:

BD LSR-II User Guide
BD LSR-II Configuration