FACS Calibur Analyzer
Description
The BD LSR-II SORP is an analyzer flow cytometer equipped with 4 lasers.
The cytometer allows the detection of 15 parameters simultaneously (15 colors plus forward scatter and side scatter).
The LSR-II SORP offers high acquisition speeds, full inter- and intra-laser compensation matrix, and multiple thresholds on all lasers. Digital pulse sampling allows the measurement of Height, Area, and Width from all parameters simultaneously.
Features
- Optical System: Patented BD OctagonTM and TrigonTM technology. A quartz cuvette is coupled to the emission lens by refractive index-matching optical gel for optimum collection efficiency.
- Laser excitation and filters (see LSR-II configuration in the related links):
- High Throughput System (HTS) – Enables analysis directly from 96 multi-well plates. The workstation is managed by FACS-Diva 6.1 software.
Additional available filters:
| Mirror | 450LP | 5005LP | 530LP | 635LP | 685LP | 735LP | ||
| Bandpass | 405/20 | 525/50 | 530/30 | 585/42 | 610/20 | 670/14 | 675/20 | 695/40 |
Acquisition Rate: >20000 events per second
Fluorescence Sensitivity: The detection limit is <200 molecules of equivalent soluble fluorochrome (MESF).
Fluorescence Resolution: The coefficient of variation (CV) of FL-Area is <3%, determined by full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN Quality Control Kit).
Fluorescence Linearity: Doublet/singlet ratio for CENs stained with PI or Hoechst is 1.95-2.05, detected with a 488 nm laser.
Forward and Side Scatter Sensitivity: Sensitivity enables the separation of fixed platelets from noise.
Signal Processing
- Digital electronics with 10 MHz sampling frequency
- 18-bit data acquisition for area signals
- Inter- and intra-beam compensation on-line and post-acquisition off-line
- Height, Width, and Area measurement available for all parameters
- Time parameter available for kinetic studies (Calcium)
- Ratio measurements between any two parameters from any laser
Software
- BD FACSDiVaTM Software Version 6.1
- All data are saved linearly
- Complex hierarchical gating for multicolor applications
- Automatic compensation of all parameters after acquisition
- Complete set of statistics for all analysis
Applications
Analysis of cells, yeast, bacteria, and beads
- Multi-color analysis
- HTS system
- Calcium Flux
- DNA analysis
Related Documents
